草業科學專用的葉綠素熒光成像系統Weed-PAM

草業科學-葉綠素熒光成像系統Weed-PAM

基于功能*的IMAG-PAM，改進升級后適用于野外雜草，草坪等光合作用研究

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主要功能

l 成像功能：對Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red等至少17種參數進行成像分析。測定調節性能量耗散Y(NPQ)，反映植物光保護能力，測定非調節性能量耗散Y(NO)，反映植物光損傷程度。

l 程序測量功能：可程序測量熒光誘導曲線、快速光曲線和暗弛豫，也可手動測量；在測量過程中能自動分析所有熒光參數的變化趨勢

l AOI功能：可在測量前或測量后任意選擇感興趣的區域（AOI），程序將自動對選擇的AOI的數據進行變化趨勢分析，并在報告文件中顯示相關AOI的數據。所有報告文件中顯示的數據都可導出到EXCEL文件中。

l 成像異質性分析功能：對任意參數任意時間的成像，可在圖像上任意選取兩點，軟件自動對兩點間的數據進行橫向異質性分析，并可導出到EXCEL文件中。

l 成像數據范圍分析功能：對任意參數任意時間的成像，可分析任意兩個熒光數值之間有多少個像素點，多少面積（cm2）。

l 突變株篩選功能：可跟據成像結果快速篩選光合、產氫/油、抗逆（抗鹽、抗旱、抗病等）等突變株。

l 吸光系數測量功能：快速測量葉片的吸光系數。吸光系數測量光源： 16個紅光（650 nm）和16個近紅外（780 nm）LED，用于測量植物葉片或藻類樣品PAR吸光系數。

成像參數

l Fo, Fm, F, Ft, Fm', Fv/Fm, Y(II), qL, qP, qN, NPQ, Y(NPQ), Y(NO), ETR, Abs, NIR和Red等

技術參數

Maxi探頭（藍光版）藍色LED，450 nm

Maxi探頭（紅光版）紅色LED，650 nm

Mini探頭（藍光版）藍色LED，460 nm

Mini探頭（紅光版）紅色LED，620 nm

應用領域

l 光合作用研究：可以在相同的條件下同時對大量樣品進行成像

l 植物病理學：病斑部位（包括肉眼不可見時）成像以及病斑擴散的時空動力學

l 植物脅迫生 理學：肉眼不可見脅迫損傷的早期檢測

l 遺傳育種：出苗后大規?？焖俸Y選高光合/抗旱/抗熱/抗凍/抗病等植株

l 突變株篩選：快速篩選模式植物的光合突變株、抗逆突變株、產氫微藻突變株等

l 分子生物學：宏觀水平上檢測樣品的綠色熒光蛋白（GFP）熒光

l 其它多種擴展研究

參考文獻

數據來源：光合作用文獻 Endnote 數據庫，更新至 2021 年 1 月，文獻數量超過 10000 篇

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